Difference between revisions of "Part:BBa K1741004"

Revision as of 13:30, 18 September 2015

sfGFP under melibiose promoter (improved 5'UTR)

Twice as high expression (comparing to BBa_K1741003) has been obtained by 5’UTR editing – removal of potential secondary structure which involved RBS and a better positioning of RBS – 7 nt upstream AUG start codon. The modified 5’UTR results in about twice higher expression upon melibiose addition to the M9 or 2xLB medium. The promoter is still weaker than araBAD or rhaBAD, so can be the promoter of choice if lower expression level is necessary for efficient folding or secretion of a recombinant protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 207

Revision as of 01:28, 18 September 2015 (view source) MartaZ (Talk \| contribs) ← Older edit		Revision as of 13:30, 18 September 2015 (view source) MartaZ (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K1741004 short</partinfo>		<partinfo>BBa_K1741004 short</partinfo>

−	Twice ~~higher~~ expression (comparing to BBa_K1741003) has been obtained by 5’UTR editing – removal of potential secondary structure which involved RBS and a better positioning of RBS – 7 nt upstream AUG start codon. The modified 5’UTR results in about twice higher expression upon melibiose addition to the M9 or 2xLB medium. The ~~promer~~ is still weaker than araBAD or rhaBAD, so can be a promoter of choice if lower expression level is necessary for efficient folding or secretion of a recombinant protein.	+	Twice as high expression (comparing to BBa_K1741003) has been obtained by 5’UTR editing – removal of potential secondary structure which involved RBS and a better positioning of RBS – 7 nt upstream AUG start codon. The modified 5’UTR results in about twice higher expression upon melibiose addition to the M9 or 2xLB medium. The promoter is still weaker than araBAD or rhaBAD, so can be the promoter of choice if lower expression level is necessary for efficient folding or secretion of a recombinant protein.