Difference between revisions of "Part:BBa K1713017:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
Aiia is a kind of enzyme could hydrolyze AHL. The RBS of Aiia is B0034. The promote LuxPR is regulated by the concentration of AHL (N-Acyl homoserine lactone). LuxI's RBS is B0034, Aiia's RBS is B0032. LVA is degradation tag.
 
Aiia is a kind of enzyme could hydrolyze AHL. The RBS of Aiia is B0034. The promote LuxPR is regulated by the concentration of AHL (N-Acyl homoserine lactone). LuxI's RBS is B0034, Aiia's RBS is B0032. LVA is degradation tag.
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===Source===
 
===Source===

Revision as of 13:29, 18 September 2015

luxpR+B0034+AiiA(+LVA)+B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 180
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Aiia is a kind of enzyme could hydrolyze AHL. The RBS of Aiia is B0034. The promote LuxPR is regulated by the concentration of AHL (N-Acyl homoserine lactone). LuxI's RBS is B0034, Aiia's RBS is B0032. LVA is degradation tag.

Source

All the basic parts come from iGEM distribution.

References