Difference between revisions of "Part:BBa K1799022"
m |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1799022 short</partinfo> | <partinfo>BBa_K1799022 short</partinfo> | ||
| − | Device was designed and assembled using Synbiota's GENtle software and Rapid DNA Prototyping (RDP) system. The device consists of | + | Device was designed and assembled using Synbiota's GENtle software and Rapid DNA Prototyping (RDP) system. The device consists of the LsrR ORF under the control of the Lac promoter and a medium-strength RBS, followed by a terminator, followed by an RFP ORF under the control of pLsrA and a medium-strength RBS. |
| − | + | ||
| − | + | ||
| − | + | ||
When cultured in a LacI- strain or in the presence of IPTG, LsrR is produced, repressing pLsrA and preventing expression of RFP. | When cultured in a LacI- strain or in the presence of IPTG, LsrR is produced, repressing pLsrA and preventing expression of RFP. | ||
Revision as of 13:28, 18 September 2015
IPTG-Repressible RFP Generator
Device was designed and assembled using Synbiota's GENtle software and Rapid DNA Prototyping (RDP) system. The device consists of the LsrR ORF under the control of the Lac promoter and a medium-strength RBS, followed by a terminator, followed by an RFP ORF under the control of pLsrA and a medium-strength RBS.
When cultured in a LacI- strain or in the presence of IPTG, LsrR is produced, repressing pLsrA and preventing expression of RFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1902
Illegal AgeI site found at 2014 - 1000COMPATIBLE WITH RFC[1000]