Difference between revisions of "Part:BBa K1736200:Design"

 
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The part contains bacerial RBA upstream of each of the four ORFs, corresponding to four subunits.
+
Four ORFs: etnA, etnB, etnC, etn D, codon-optimised for expression in ''Pseudomonas'', each ORF has native RBS upstream
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 13:09, 18 September 2015


Harmonised Ethene Monooxygenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 554
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 119
    Illegal BglII site found at 3698
    Illegal BamHI site found at 254
    Illegal BamHI site found at 969
    Illegal BamHI site found at 1887
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 146
    Illegal NgoMIV site found at 1717
    Illegal NgoMIV site found at 2286
    Illegal NgoMIV site found at 2825
    Illegal NgoMIV site found at 3246
    Illegal AgeI site found at 3949
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Four ORFs: etnA, etnB, etnC, etn D, codon-optimised for expression in Pseudomonas, each ORF has native RBS upstream

Source

Harmonised sequence (via codon harmonisation algorithm) of the Mycobacterium chubeunse ethene monooxygenase enzyme.

References