Difference between revisions of "Part:BBa K1638009:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This part was assemblied by the part containing the T18 domain (<partinfo>BBa_K1638005</partinfo>) and the linker-GFP <partinfo>BBa_K1638007</partinfo>. These parts respectively contains a BamHI res. site in the 3'-end and the 5'-end allowing assembly without creating a scarsite. Scarsite includes a stop-codon, which is not favorable for correct of assembly of fusion proteins.
+
This part was assemblied by the part containing the T18 domain (<partinfo>BBa_K1638005</partinfo>) and the linker-GFP (<partinfo>BBa_K1638007</partinfo>). These parts respectively contains a BamHI res. site in the 3'-end and the 5'-end allowing assembly without creating a scarsite. Scarsite includes a stop-codon, which is not favorable for correct of assembly of fusion proteins.
  
 
===Source===
 
===Source===

Revision as of 12:52, 18 September 2015


T18 domain of cyaA from Bordetella pertussis with GFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 711
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 252
    Illegal NgoMIV site found at 662
    Illegal AgeI site found at 468
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1384


Design Notes

This part was assemblied by the part containing the T18 domain (BBa_K1638005) and the linker-GFP (BBa_K1638007). These parts respectively contains a BamHI res. site in the 3'-end and the 5'-end allowing assembly without creating a scarsite. Scarsite includes a stop-codon, which is not favorable for correct of assembly of fusion proteins.

Source

..

References