Difference between revisions of "Part:BBa K1736000:Design"
(→References) |
(→Source) |
||
| Line 11: | Line 11: | ||
===Source=== | ===Source=== | ||
| − | Bacillus Subtilits | + | ''Bacillus Subtilits'' |
===References=== | ===References=== | ||
<sup>1</sup> Spencer P et al., 2012, “Silent Substitutions Predictably Alter Translation Elongation Rates and Protein Folding Efficiencies”, Vol 422, pp. 328-335. | <sup>1</sup> Spencer P et al., 2012, “Silent Substitutions Predictably Alter Translation Elongation Rates and Protein Folding Efficiencies”, Vol 422, pp. 328-335. | ||
Revision as of 12:48, 18 September 2015
Harmonised B. subtilis Falvin Binding Fluorescent Protein (BsFP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 109
Illegal BamHI site found at 229
Illegal XhoI site found at 425 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The BsFP protein was harmonised by the method outlined in Spencer et al. 1. To download and obtain instructions on how to use the algorithm to harmonise your sequence, refer to the Sydney 2015 [http://2015.igem.org/Team:Sydney_Australia/Modeling modelling page]. Note that the part also contains an B.subtilis RBS.
Source
Bacillus Subtilits
References
1 Spencer P et al., 2012, “Silent Substitutions Predictably Alter Translation Elongation Rates and Protein Folding Efficiencies”, Vol 422, pp. 328-335.