Difference between revisions of "Part:BBa K1736000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Note that the part also contains an E. coli RBS.
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The BsFP protein was harmonised by the method outlined in Spencer ''et al.'' <sup>1</sup> Note that the part also contains an ''B.subtilis'' RBS.
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<sup>1</sup> Spencer P et al., 2012, “Silent Substitutions Predictably Alter Translation Elongation Rates and Protein Folding Efficiencies”, Vol 422, pp. 328-335.
  
 
===Source===
 
===Source===

Revision as of 12:44, 18 September 2015


Harmonised B. subtilis Falvin Binding Fluorescent Protein (BsFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 109
    Illegal BamHI site found at 229
    Illegal XhoI site found at 425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The BsFP protein was harmonised by the method outlined in Spencer et al. 1 Note that the part also contains an B.subtilis RBS.

1 Spencer P et al., 2012, “Silent Substitutions Predictably Alter Translation Elongation Rates and Protein Folding Efficiencies”, Vol 422, pp. 328-335.

Source

Bacillus Subtilits

References