Difference between revisions of "Part:BBa K1638010"
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T25 domain of the catalytic active domain of CyaA from <i>Bordetella pertussis</i> linked together with green fluorescent protein (GFP) through a flexible 10 aa linker. This part allows detection of expression of the gene encoding the T25-GFP fusion protein. Additionally, the presence of green fluorescence will verify correct folding of the protein fused to the T25-domain through the flexible aa linker. Correct folding of our T18-domain is obviously improtant for the bacterial two-hybrid system to work. | T25 domain of the catalytic active domain of CyaA from <i>Bordetella pertussis</i> linked together with green fluorescent protein (GFP) through a flexible 10 aa linker. This part allows detection of expression of the gene encoding the T25-GFP fusion protein. Additionally, the presence of green fluorescence will verify correct folding of the protein fused to the T25-domain through the flexible aa linker. Correct folding of our T18-domain is obviously improtant for the bacterial two-hybrid system to work. | ||
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Revision as of 12:37, 18 September 2015
T25 domain of cyaA from Bordetella pertussis with GFP reporter
T25 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with green fluorescent protein (GFP) through a flexible 10 aa linker. This part allows detection of expression of the gene encoding the T25-GFP fusion protein. Additionally, the presence of green fluorescence will verify correct folding of the protein fused to the T25-domain through the flexible aa linker. Correct folding of our T18-domain is obviously improtant for the bacterial two-hybrid system to work.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 843
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1516