Difference between revisions of "Part:BBa K1859026"
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His-RFPの配列を含むmRNAを環状化させる効率を上げるために、私たちはこのジェネレーター[K1859026]をデザインした。
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We designed this generator (K1859026) to improve circular efficiency of mRNA including His-RFP sequence.
 
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The generator consists of 相補的になるように改良したCircular parts (
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The generator is comprised of Circular parts improved to become complementary (
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859015" > [BBa_K1859015] </a>
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859015" > [BBa_K1859015] </a>
 
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このジェネレーター[BBa_1859026]で作成したRNAは環状mRNAの外側が相補的に結合するように設計されている。
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Tne RNA made by this generator (BBa_1859026) was designed to bind outside of circular mRNA complementary.
 
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iGEM2014のジェネレーター[BBa_1332011]で作成した環状mRNAの量、[BBa_1859024]で作成した環状mRNAの量、[BBa_1859025]で作成した環状mRNAの量、[BBa_1859026]で作成した環状mRNAの量を比較すると、環状化の効率は[BBa_1859026]が最も良かった。
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We found that circular efficiency of [BBa_1859026] was the best,  when we compared with amount of circular mRNA made by these generators (BBa_1332011), (BBa_1859024), (BBa_18559025), (BBa_1859026).</p>
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Revision as of 12:34, 18 September 2015

mRNA circularize efficiently generator (outside complementarity)

We designed this generator (K1859026) to improve circular efficiency of mRNA including His-RFP sequence.

First, we improved Circular Parts of iGEM2014,the 5´side of the intron [BBa_K1332003] and the 3´side of the intron [BBa_K1332005] , and made BBa_K1859015 and BBa_K1859016. Circular parts of iGEM2014 were cloned only sequence of ribozyme involved the splicing from td intron in T4 phage. However, BBa_K1859015 and BBa_K1859016 were cloned the sequence except the ribozyme extra in addition to [BBa_K1332003] and [BBa_K1332005] .

The generator is comprised of Circular parts improved to become complementary ( [BBa_K1859015] and [BBa_K1859016] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .

Tne RNA made by this generator (BBa_1859026) was designed to bind outside of circular mRNA complementary.

We found that circular efficiency of [BBa_1859026] was the best, when we compared with amount of circular mRNA made by these generators (BBa_1332011), (BBa_1859024), (BBa_18559025), (BBa_1859026).