Difference between revisions of "Part:BBa K1807000:Design"
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BBa_K1807000 Assembly 3'end Overhang (complementary to pSB1C3): TACTAGTAGCGGCCGCTGCAGtccggcaaaaaagggcaag | BBa_K1807000 Assembly 3'end Overhang (complementary to pSB1C3): TACTAGTAGCGGCCGCTGCAGtccggcaaaaaagggcaag | ||
| + | |||
| + | |||
| + | '''The subparts of the device are as follows:''' | ||
| + | BBa_R0011, | ||
| + | BBa_B0034, | ||
| + | BBa_E0038, | ||
| + | BBa_B0015. | ||
Revision as of 12:32, 18 September 2015
BBa_K1807000 was designed as a blue-white screening device that would also be easily used in Gibson Assembly. Surrounding the lacZ alpha coding sequence are two SmaI restriction sites (CCC/GGG). SmaI is a blunt-end endonuclease- we used it to simulatenously linearize our vector and remove the lacZ alpha coding sequence. Our BioBricks contained overhangs that make them compatible with the SmaI-digested BBa_K1807000.
The Overhang Sequences are as follows:
BBa_K1807000 Assembly 5'end Overhang (complementary to pSB1C3): cgctaaggatgatttctgGAATTCGCGGCCGCTTCTAGAG
BBa_K1807000 Assembly 3'end Overhang (complementary to pSB1C3): TACTAGTAGCGGCCGCTGCAGtccggcaaaaaagggcaag
The subparts of the device are as follows:
BBa_R0011,
BBa_B0034,
BBa_E0038,
BBa_B0015.