Difference between revisions of "Part:BBa K1813063"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1813063 short</partinfo>
 
<partinfo>BBa_K1813063 short</partinfo>
  
 
ptac pelB AgCPR pelb HUMCYPDB1
 
ptac pelB AgCPR pelb HUMCYPDB1
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With ptac powering the transcription of <html><b> <a href="https://parts.igem.org/Part:BBa_K1813053">BBa_K1813053</a></b></html> and <html><b> <a href="https://parts.igem.org/Part:BBa_K1813055">BBa_K1813055</a></b></html>, this part allows the co-localization of large amounts of simultaneously expressed HUMCYPDB1 (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</a></b></html>) and <i>A. gambiae</i> NADPH p450 reductase (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</a></b></html>) to the bacterial periplasm.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:19, 18 September 2015

ptac pelB AgCPR pelb CYP2D6

ptac pelB AgCPR pelb HUMCYPDB1

With ptac powering the transcription of BBa_K1813053 and BBa_K1813055, this part allows the co-localization of large amounts of simultaneously expressed HUMCYPDB1 ( BBa_K1813005) and A. gambiae NADPH p450 reductase ( BBa_K1813010) to the bacterial periplasm.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2783
    Illegal BamHI site found at 626
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 141
    Illegal NgoMIV site found at 2448
    Illegal NgoMIV site found at 3373
    Illegal AgeI site found at 424
    Illegal AgeI site found at 1606
    Illegal AgeI site found at 1762
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2122