Difference between revisions of "Part:BBa K1593209"
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OprF is a kind of porin on the outer membrane of Pseudomonus aeruginosa. OprF represents one of the largest pore sizes on bacterial outer membranes, allowing diffusion of polysaccharides in a range of 2000 to 3000 Da. The overexpression of OprF in E. coli will drastically improve the antibiotic absorption capability, like antibiotic condensation occured in E. coli. | OprF is a kind of porin on the outer membrane of Pseudomonus aeruginosa. OprF represents one of the largest pore sizes on bacterial outer membranes, allowing diffusion of polysaccharides in a range of 2000 to 3000 Da. The overexpression of OprF in E. coli will drastically improve the antibiotic absorption capability, like antibiotic condensation occured in E. coli. | ||
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| − | [[ | + | == electrophorogram == |
| + | [[Media:https://static.igem.org/mediawiki/2015/6/66/USTC-OprF.png]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:10, 18 September 2015
Porin: OprF from Pseudomonas aeruginosa
OprF is a kind of porin on the outer membrane of Pseudomonus aeruginosa. OprF represents one of the largest pore sizes on bacterial outer membranes, allowing diffusion of polysaccharides in a range of 2000 to 3000 Da. The overexpression of OprF in E. coli will drastically improve the antibiotic absorption capability, like antibiotic condensation occured in E. coli.
electrophorogram
Media:https://static.igem.org/mediawiki/2015/6/66/USTC-OprF.png
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 582
- 1000COMPATIBLE WITH RFC[1000]