Difference between revisions of "Part:BBa K1686033:Experience"
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===Applications of BBa_K1686033=== | ===Applications of BBa_K1686033=== | ||
| − | To verify <i>OsmY</i> promoter is only active in | + | To verify that the <i>OsmY</i> promoter is only active in stationary phase, we realized Curdlan quantitative analyses every hour of a culture in LB medium. We could therefore show a parallel between growth and Curdlan production. The culture temperature was changed when stationary phase was obtained. |
<br>→ As we can see, Curdlan appears after the switch at 27°C. So, <i>OsmY</i> promoter is active in stationary phase only. | <br>→ As we can see, Curdlan appears after the switch at 27°C. So, <i>OsmY</i> promoter is active in stationary phase only. | ||
https://static.igem.org/mediawiki/2015/thumb/1/16/Bordeaux_Team_promoter_characterizationV4.png/800px-Bordeaux_Team_promoter_characterizationV4.png | https://static.igem.org/mediawiki/2015/thumb/1/16/Bordeaux_Team_promoter_characterizationV4.png/800px-Bordeaux_Team_promoter_characterizationV4.png | ||
| − | <b> Fig.1: <i>OsmY</i> promoter characterization</B> | + | <b> Fig.1: <i>OsmY</i> promoter characterization</B> : The production of Curdlan was made in LB medium at 37°C during exponential phase and after a temperature switch at 27°C at the beginning of the stationary phase (to slow down cell metabolism). The Curdlan concentration was calculated with absorption measures (excitation wavelength at 398nm and emission wavelength= 502nm) after Curdlan purification from cells. We used aniline blue dye since it is specific of beta glucans. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 12:08, 18 September 2015
Applications of BBa_K1686033
To verify that the OsmY promoter is only active in stationary phase, we realized Curdlan quantitative analyses every hour of a culture in LB medium. We could therefore show a parallel between growth and Curdlan production. The culture temperature was changed when stationary phase was obtained.
→ As we can see, Curdlan appears after the switch at 27°C. So, OsmY promoter is active in stationary phase only.
Fig.1: OsmY promoter characterization : The production of Curdlan was made in LB medium at 37°C during exponential phase and after a temperature switch at 27°C at the beginning of the stationary phase (to slow down cell metabolism). The Curdlan concentration was calculated with absorption measures (excitation wavelength at 398nm and emission wavelength= 502nm) after Curdlan purification from cells. We used aniline blue dye since it is specific of beta glucans.
User Reviews
UNIQb068334230708322-partinfo-00000000-QINU UNIQb068334230708322-partinfo-00000001-QINU