Difference between revisions of "Part:BBa K1789022"

Revision as of 11:41, 18 September 2015

IAA(+/+)S2

This is a device which contains the sequence of the recombination of TALE1 and IAAM, the recombination of TALE2 and IAAH and scaffold 2. Through this device, we can test and verify that our scaffold can strengthen the function of IAA.

Usage and Biology

Indole-3-acetic acid (IAA), also known as auxin, is a plant hormone, which can promote growth and protect the soil from erosion. IAA can be synthesized through IAM pathway, originated from Pseudomonas savastanoi. Two important enzymes are contained in this pathway, AKA IaaM and IaaH.

In this device, the TALE1-fused IAAM and the TALE2-fused IAAH are incorporated in the E.coli due to the specific binding of TALE proteins and scaffold2, which could efficiently guide the enzymes around the DNA scaffolds, to enrich the local enzyme concentration, and promote the rate of reaction.

Sequence and Features

Sequence and Features

Experimental Validation