Difference between revisions of "Part:BBa K1633004:Experience"

 
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===Applications of BBa_K1633004===
 
===Applications of BBa_K1633004===
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==== USAGE AND BIOLOGY ====
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We package MOR siRNA into exosomes by transfecting HEK293 cells with a a Lamp2b-RVG plasmid and the MOR siRNA-2 plasmid and then collect siRNA-encapsulated exosomes. When inject the modified exosomes into the bloodstream, exosome will specifically recognize acetylcholine receptors and fuse with neurons under the direction of the RVG peptide. Once inside neurons, MOR siRNA will degrade MOR mRNA by base-pairing, resulting in sharp decrease of MOR on neuron membrane. As a consequence, MOR reduction and disturbed function will result in the inhabitation of the secretion of GABA and the suppression of the dopaminergic reward pathway, which ultimately have some therapeutic effects on opioid dependence.
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==== CHARACTERIZATION ====
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====== Interference efficiency of MOR siRNA-2 plasmid ======
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To ensure the interference efficiency,MOR siRNA-2 plasmid was transfected into the mouse neuroblastoma cell line Neuro2A. Efficient knockdown of MOR by MOR siRNA-2 in Neuro2A cells is observed.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 11:32, 18 September 2015


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Applications of BBa_K1633004

USAGE AND BIOLOGY

We package MOR siRNA into exosomes by transfecting HEK293 cells with a a Lamp2b-RVG plasmid and the MOR siRNA-2 plasmid and then collect siRNA-encapsulated exosomes. When inject the modified exosomes into the bloodstream, exosome will specifically recognize acetylcholine receptors and fuse with neurons under the direction of the RVG peptide. Once inside neurons, MOR siRNA will degrade MOR mRNA by base-pairing, resulting in sharp decrease of MOR on neuron membrane. As a consequence, MOR reduction and disturbed function will result in the inhabitation of the secretion of GABA and the suppression of the dopaminergic reward pathway, which ultimately have some therapeutic effects on opioid dependence.

CHARACTERIZATION

Interference efficiency of MOR siRNA-2 plasmid

To ensure the interference efficiency,MOR siRNA-2 plasmid was transfected into the mouse neuroblastoma cell line Neuro2A. Efficient knockdown of MOR by MOR siRNA-2 in Neuro2A cells is observed.

User Reviews

UNIQ530a682de495b230-partinfo-00000000-QINU UNIQ530a682de495b230-partinfo-00000001-QINU