Difference between revisions of "Part:BBa K1633003:Experience"
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To ensure the interference efficiency, MOR siRNA-1 plasmid was,transfected into the mouse neuroblastoma cell line Neuro2A. Efficient knockdown of MOR by MOR siRNA-1 in Neuro2A cells is observed. | To ensure the interference efficiency, MOR siRNA-1 plasmid was,transfected into the mouse neuroblastoma cell line Neuro2A. Efficient knockdown of MOR by MOR siRNA-1 in Neuro2A cells is observed. | ||
| − | [[File:NJU-China-parts-fig 2.png| | + | [[File:NJU-China-parts-fig 2.png|400px]] |
Figure 2. Relative level of MOR mRNA in Neuro2A cell after transfection of MOR siRNA-1 plasmids. | Figure 2. Relative level of MOR mRNA in Neuro2A cell after transfection of MOR siRNA-1 plasmids. | ||
Revision as of 11:28, 18 September 2015
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how you used this part and how it worked out.
Applications of BBa_K1633003
Usage and Biology
This part is a shRNA designed to target and degrade MOR mRNA. When this shRNA sequence is cut by restriction enzyme and then integrated into mammalian vector, this shRNA can play a RNAi function in mammalian cell lines. When the shRNA vector of MOR is transfected into mammalian cells, the shRNA hairpin structure is cleaved by Dicer into siRNA of MOR and loaded into the RISC. The siRNA-RISC complex targets at MOR mRNA under the guide of siRNA sequence and cleave the MOR mRNA.
CHARACTERIZATION
Interference efficiency of MOR siRNA-1 plasmid
To ensure the interference efficiency, MOR siRNA-1 plasmid was,transfected into the mouse neuroblastoma cell line Neuro2A. Efficient knockdown of MOR by MOR siRNA-1 in Neuro2A cells is observed.
Figure 2. Relative level of MOR mRNA in Neuro2A cell after transfection of MOR siRNA-1 plasmids.
User Reviews
UNIQdcf9813a74218a4a-partinfo-00000000-QINU UNIQdcf9813a74218a4a-partinfo-00000001-QINU