Difference between revisions of "Part:BBa K1633003:Experience"

Revision as of 11:20, 18 September 2015

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Please enter how you used this part and how it worked out.

Applications of BBa_K1633003

CHARACTERIZATION

Interference efficiency of MOR siRNA-1 plasmid

To ensure the interference efficiency, MOR siRNA-1 plasmid was,transfected into the mouse neuroblastoma cell line Neuro2A. Efficient knockdown of MOR by MOR siRNA-1 in Neuro2A cells is observed.

Figure 2. Relative level of MOR mRNA in Neuro2A cell after transfection of MOR siRNA-1 plasmids.

User Reviews

UNIQ72715dc77b3e42b8-partinfo-00000000-QINU UNIQ72715dc77b3e42b8-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K1633003===
-'''INTRODUCTION'''
+== CHARACTERIZATION ==
-This part is an artificially designed RNA strand. It serves as an element of the Team NJU-CHINA RNAi module. We use it as the siRNA medicine to downregulate the expression of Mu opioid receptor in brain tissue. We designed specific MOR siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene MOR to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid. Then we synthesize the shRNA sequences with the help of a DNA synthesis company. We totally got four such shRNA plasmids.Although MOR siRNA-1 plasmid can efficiently knock down MOR expression, it does not show best interference efficiency and therefore serve as a backup.
+Interference efficiency of MOR siRNA-1 plasmid
-[[File:NJU-China-parts-parts1.png|500px|]]
+To ensure the interference efficiency, MOR siRNA-1 plasmid was,transfected into the mouse neuroblastoma cell line Neuro2A. Efficient knockdown of MOR by MOR siRNA-1 in Neuro2A cells is observed.
+[[File:NJU-China-parts-fig 2.png|200px]]
+Figure 2. Relative level of MOR mRNA in Neuro2A cell after transfection of MOR siRNA-1 plasmids.
 ===User Reviews===