Difference between revisions of "Part:BBa K1789005:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We designed this sequence to provide sites for our engineered TALE proteins to bind
 
  
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The length of DNA BMs and intervening sequences is designed based on the typical in vivo B-type DNA structure, and the Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.
  
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This scaffold is designed to put enzymes as close as possible, no intervening sequence is inserted between two BMs
  
 
===Source===
 
===Source===

Revision as of 11:07, 18 September 2015


SCAF1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 8
    Illegal AgeI site found at 38
    Illegal AgeI site found at 68
    Illegal AgeI site found at 98
    Illegal AgeI site found at 128
    Illegal AgeI site found at 158
    Illegal AgeI site found at 188
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The length of DNA BMs and intervening sequences is designed based on the typical in vivo B-type DNA structure, and the Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.

This scaffold is designed to put enzymes as close as possible, no intervening sequence is inserted between two BMs

Source

This part is synthesized by company

References