Difference between revisions of "Part:BBa K1797000"

Revision as of 11:06, 18 September 2015

Blue Light Sensing System with GFP Reporter

This part is a combination of B0034-YF1-B0034-FixJ(BBa_K592016), FixK2 promoter(BBa_K592006), and amilGFP(BBa_K592010) in that order. We also add a constitutive promoter(BBa_J23119) so that teams can use this part to test the blue light system easily. When the cells are kept in dark, they will express amilGFP, a yellow chromoprotein. This protein causes the cells to have a yellow color. When the cells are exposed to blue light, the amilGFP won't be expressed and the yellow color can't be seen.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 605
Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 767
Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 1590
Illegal NgoMIV site found at 1684
Illegal AgeI site found at 319
Illegal AgeI site found at 1465
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1354
Illegal BsaI.rc site found at 218

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1797000 short</partinfo>
-This part is a combination of BBa_K592016, BBa_K592006, and BBa_K592010 in that order. We also add a promoter called promoter 104 so that teams can use this part to test the blue light system easily. When the cells are kept in dark, they will express amilGFP, a yellow chromoprotein. This protein causes the cells to have a yellow color. When the cells are exposed to blue light, the amilGFP won't be expressed and the yellow color can't be seen.
+This part is a combination of B0034-YF1-B0034-FixJ(BBa_K592016), FixK2 promoter(BBa_K592006), and amilGFP(BBa_K592010) in that order. We also add a constitutive promoter(BBa_J23119) so that teams can use this part to test the blue light system easily. When the cells are kept in dark, they will express amilGFP, a yellow chromoprotein. This protein causes the cells to have a yellow color. When the cells are exposed to blue light, the amilGFP won't be expressed and the yellow color can't be seen.
 <!-- Add more about the biology of this part here123456