Difference between revisions of "Part:BBa K1734004"
 
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Nluc luciferase is an enzyme that was engineered to work as a luminescent reporter weighing 19.1 kDa. This protein uses fumarizine as novel substrate to produce a high intensity glow-type luminescence. These reactions are ATP-independent, trying to obtain the maximal assay sensitivity.   
 
Nluc luciferase is an enzyme that was engineered to work as a luminescent reporter weighing 19.1 kDa. This protein uses fumarizine as novel substrate to produce a high intensity glow-type luminescence. These reactions are ATP-independent, trying to obtain the maximal assay sensitivity.   
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https://static.igem.org/mediawiki/2015/f/f2/Tec_Monterrey_Conf_Nanluc.jpg
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This part has been confirmed by restriction analysis of the pSB1C3 vector
  
  

Latest revision as of 10:48, 18 September 2015

Nanoluc (Codon optimized for Sf9 cells)

Nluc luciferase is an enzyme that was engineered to work as a luminescent reporter weighing 19.1 kDa. This protein uses fumarizine as novel substrate to produce a high intensity glow-type luminescence. These reactions are ATP-independent, trying to obtain the maximal assay sensitivity.


Tec_Monterrey_Conf_Nanluc.jpg

This part has been confirmed by restriction analysis of the pSB1C3 vector


Reference:

[1] Promega (2015). Nanoluc Genetic Reporter Vectors. URL: http://worldwide.promega.com/products/reporter-assays-and-transfection/reporter-vectors-and-cell-lines/nanoluc-luciferase-reporters/nanoluc-genetic-reporter-vectors/


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 458