Difference between revisions of "Part:BBa K1734003"

 
Line 4: Line 4:
 
The biobrick contains the polyhedrin promoter from the baculovirus Autographa californica. After the promoter there is a secretion signal sequence of the protein lysozyme from the organism Gallus gallus (chicken) [1].
 
The biobrick contains the polyhedrin promoter from the baculovirus Autographa californica. After the promoter there is a secretion signal sequence of the protein lysozyme from the organism Gallus gallus (chicken) [1].
  
 +
https://static.igem.org/mediawiki/2015/4/41/Tec_Monterrey_Conf_PPH_CL.jpg
  
 +
This part has been confirmed by restriction analysis of the pSB1C3 vector
  
 
Reference:
 
Reference:

Latest revision as of 10:44, 18 September 2015

Polyhedrin promoter + Lysozymes signal peptide

The biobrick contains the polyhedrin promoter from the baculovirus Autographa californica. After the promoter there is a secretion signal sequence of the protein lysozyme from the organism Gallus gallus (chicken) [1].

Tec_Monterrey_Conf_PPH_CL.jpg

This part has been confirmed by restriction analysis of the pSB1C3 vector

Reference:

[1]Tsuchiya, Y., Morioka, K., Shirai, J., & Yoshida, K. (2004). Structural requirements of signal peptide in insect cell. Nucleic Acids Symposium Series, 48(1), 181-182.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]