Difference between revisions of "Part:BBa K1640022"

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<partinfo>BBa_K1640022 short</partinfo>
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1640022 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1640022 SequenceAndFeatures</partinfo>
 
 
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===Functional Parameters===
 
<partinfo>BBa_K1640022 parameters</partinfo>
 
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===Overview===
 
===Overview===

Revision as of 10:40, 18 September 2015

psbP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 537
  • 1000
    COMPATIBLE WITH RFC[1000]

Overview

This biobrick contains a gene from the photosystem II complex of Chlamydomonas reinhardtii - psbP, with RBS included.

This part was designed in conjunction with BBa_K1640012 and BBa_K1640023, together they form operon 5 in our set of photosystem II operons:

PSII diagram

Biology & Literature

The psbP gene that comprises this part transcribes a protein that optimizes the availability of Ca2+ and Cl- cofactors in the Oxygen Evolving Complex in PSII to maintain the active Manganese cluster.

Protein information

psbP

mass: 23.17kDa

sequence:
MASGSDVSRRAALAGFAGAAALVSSSPANAAYGDSANVFGKVTNKSGFVPYAGDGFALLLPAKWNPSKENDF PGVILRYEDNFDAVNNLVVIAQDTDKKAIADFGSQDKFLESVSYLLGKQAYSGETQSEGGFAPNRVSAASLL DVSTTTDKKGKTYYKYELLVRSADGDEGGRHQLIGATVGSDNKLYIIKIQIGDKRWFKGAKKEAMGAFDSFT VV*


References