Difference between revisions of "Part:BBa K1638038:Design"
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===References=== | ===References=== | ||
| + | [1]: Karimova G, Pidoux J, Ullmann A, Ladant D. A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(10):5752-6. | ||
Latest revision as of 10:39, 18 September 2015
Yhbj fused to T25 domain of CyaA with cAMP-induced RFP reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1851
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 712
Illegal AgeI site found at 824 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The fragment of this part containing Plac-T25-yhbJ was synthesized by IDT as a gBlock gene fragment. Standard BioBrick assembly was used to construct the whole device including the mRFP generator controlled by PcstA (BBa_K861173).
Source
pKT25
yhbJ: Gene ID: 947727
References
[1]: Karimova G, Pidoux J, Ullmann A, Ladant D. A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(10):5752-6.