Difference between revisions of "Part:BBa K1638039:Design"

(→‎Design Notes)
(→‎Source)
Line 10: Line 10:
  
 
===Source===
 
===Source===
−
 
+
pKT25
−
.
+
<br>
 +
<i>hfq</i>: Gene ID: 948689
  
 
===References===
 
===References===

Revision as of 10:34, 18 September 2015


Hfq fused to T25 domain of CyaA with cAMP-induced RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1851
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 712
    Illegal AgeI site found at 824
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fragment of this part containing Plac-T25-hfq was synthesized by IDT as a gBlock gene fragment. Standard BioBrick assembly was used to construct the whole device including the mRFP generator controlled by PcstA (BBa_K861173).

Source

pKT25
hfq: Gene ID: 948689

References