Difference between revisions of "Part:BBa K1638039:Design"

 
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
.
+
The fragment of this part containing Plac-T25-<i>hfq</i> was synthesized by IDT as a gBlock gene fragment. Standard BioBrick assembly was used to construct the whole device including the mRFP generator controlled by PcstA (<partinfo>BBa_K861173</partinfo>).
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 10:33, 18 September 2015


Hfq fused to T25 domain of CyaA with cAMP-induced RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1851
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 712
    Illegal AgeI site found at 824
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fragment of this part containing Plac-T25-hfq was synthesized by IDT as a gBlock gene fragment. Standard BioBrick assembly was used to construct the whole device including the mRFP generator controlled by PcstA (BBa_K861173).

Source

.

References