Difference between revisions of "Part:BBa K1789006"

 
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<partinfo>BBa_K1789006 short</partinfo>
 
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This part is a highly repetitive DNA sequence which can bind TALE protein specifically.
 
This part is a highly repetitive DNA sequence which can bind TALE protein specifically.
  
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==Usage and Biology==
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As is mentioned in our project description, different TALEs share a similar domain structure that enables them to bind the genome of the host cell and act as transcriptional effectors. By engineering those structures, we can build proteins that can bind with any DNA sequence that we desire.
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In our project, we designed two different DNA binding motifs (DNA BMs). The sequences were chosen from Danio rerio CD154 gene in order to avoid homology with E.coli genome. Those BMs are sequenced as
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BM1: 5’-GGAGGCACCGGTGG-3’
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MB2: 5’-GATAAACACCTTTC-3’
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Those sequences were repeated for more than 10 times in a plasmid, with different length of intervening sequence.
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[[File:SCAF2_A.jpg|500px|]]
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This scaffold is designed to put enzymes as close as possible, no intervening sequence is inserted between two BMs.
  
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===Usage and Biology===
 
  
 
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Revision as of 10:33, 18 September 2015

SCAF2

This part is a highly repetitive DNA sequence which can bind TALE protein specifically.

Usage and Biology

As is mentioned in our project description, different TALEs share a similar domain structure that enables them to bind the genome of the host cell and act as transcriptional effectors. By engineering those structures, we can build proteins that can bind with any DNA sequence that we desire.

In our project, we designed two different DNA binding motifs (DNA BMs). The sequences were chosen from Danio rerio CD154 gene in order to avoid homology with E.coli genome. Those BMs are sequenced as

BM1: 5’-GGAGGCACCGGTGG-3’

MB2: 5’-GATAAACACCTTTC-3’

Those sequences were repeated for more than 10 times in a plasmid, with different length of intervening sequence.

SCAF2 A.jpg

This scaffold is designed to put enzymes as close as possible, no intervening sequence is inserted between two BMs.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 8
    Illegal AgeI site found at 50
    Illegal AgeI site found at 92
    Illegal AgeI site found at 134
    Illegal AgeI site found at 176
    Illegal AgeI site found at 218
    Illegal AgeI site found at 260
  • 1000
    COMPATIBLE WITH RFC[1000]