Difference between revisions of "Part:BBa K1638038:Design"

 
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===Design Notes===
 
===Design Notes===
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The fragment of this part containing Plac-T25-<i>yhbJ</i> was synthesized by IDT as a gBlock gene fragment. Standard BioBrick assembly was used to construct the whole device including the mRFP generator controlled by PcstA (<partinfo>BBa_K861173</partinfo>).
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===Source===
 
===Source===

Revision as of 10:30, 18 September 2015


Yhbj fused to T25 domain of CyaA with cAMP-induced RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1851
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 712
    Illegal AgeI site found at 824
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fragment of this part containing Plac-T25-yhbJ was synthesized by IDT as a gBlock gene fragment. Standard BioBrick assembly was used to construct the whole device including the mRFP generator controlled by PcstA (BBa_K861173).

Source

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References