Difference between revisions of "Part:BBa K1638037:Design"
Jpettersen (Talk | contribs) (→Design Notes) |
Jpettersen (Talk | contribs) (→Design Notes) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | The fragment of this part containing Plac-T25-CrsA was synthesized by IDT as a gBlock gene fragment. Standard BioBrick assembly was used to construct the whole device including the mRFP generator controlled by PcstA (<partinfo>BBa_K861173</partinfo>. | + | The fragment of this part containing Plac-T25-CrsA was synthesized by IDT as a gBlock gene fragment. Standard BioBrick assembly was used to construct the whole device including the mRFP generator controlled by PcstA (<partinfo>BBa_K861173</partinfo>). |
===Source=== | ===Source=== | ||
Revision as of 10:25, 18 September 2015
CsrA fused to T25 domain of CyaA with cAMP-induced RFP reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1851
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 712
Illegal AgeI site found at 824 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The fragment of this part containing Plac-T25-CrsA was synthesized by IDT as a gBlock gene fragment. Standard BioBrick assembly was used to construct the whole device including the mRFP generator controlled by PcstA (BBa_K861173).
Source
.