Difference between revisions of "Part:BBa K1642001:Design"

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<partinfo>BBa_K1642001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1642001 SequenceAndFeatures</partinfo>
 
 
===Design Notes===
 
restriction
 
 
 
  
 
===Source===
 
===Source===
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===References===
 
===References===
[1]Amezaga, J. M., Amtmann, A., Biggs, C. A., Bond, T., Gandy, C. J., Honsbein, A., ... & Templeton, M. R. (2014). Biodesalination: a case study for applications of photosynthetic bacteria in water treatment. Plant physiology, 164(4), 1661-1676.
+
''Amezaga, J. M., Amtmann, A., Biggs, C. A., Bond, T., Gandy, C. J., Honsbein, A., ... & Templeton, M. R. (2014). Biodesalination: a case study for applications of photosynthetic bacteria in water treatment. Plant physiology, 164(4), 1661-1676.
  
[2]Seki, A., Miyauchi, S., Hayashi, S., Kikukawa, T., Kubo, M., Demura, M., ... & Kamo, N. (2007). Heterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl− pump activity. Biophysical journal, 92(7), 2559-2569.
+
Seki, A., Miyauchi, S., Hayashi, S., Kikukawa, T., Kubo, M., Demura, M., ... & Kamo, N. (2007). Heterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl− pump activity. Biophysical journal, 92(7), 2559-2569.''

Revision as of 10:21, 18 September 2015

Codon-optimized Halorhodopsin with His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 133
    Illegal NgoMIV site found at 292
    Illegal NgoMIV site found at 613
    Illegal AgeI site found at 484
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Synthesized

References

Amezaga, J. M., Amtmann, A., Biggs, C. A., Bond, T., Gandy, C. J., Honsbein, A., ... & Templeton, M. R. (2014). Biodesalination: a case study for applications of photosynthetic bacteria in water treatment. Plant physiology, 164(4), 1661-1676.

Seki, A., Miyauchi, S., Hayashi, S., Kikukawa, T., Kubo, M., Demura, M., ... & Kamo, N. (2007). Heterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl− pump activity. Biophysical journal, 92(7), 2559-2569.