Difference between revisions of "Part:BBa K1642001"
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Revision as of 10:20, 18 September 2015
Codon-optimized Halorhodopsin with His-tag
Halorhodopsin (HR) proteins are light-driven inward-directed chloride pumps from halobacteria. They are membrane-integral proteins of the rhodopsin superfamily that form a covalent bond with the carotenoid-derived chromophore all-trans retinal. Absorption of a photon with a defined optimal wavelength induces trans-cis isomerization of retinal, which triggers a catalytic photocycle of conformational changes in the protein, resulting in the net import of one chloride per photon into the cytoplasm(Amezaga, J. M. et al. 2014) . We use it as our biodesalination driver which confers cyanobacteria the ability to absorb chloride to a significant degree.
His-tag is used to produce anti-His mAb as a first antibody or to purify the protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 133
Illegal NgoMIV site found at 292
Illegal NgoMIV site found at 613
Illegal AgeI site found at 484 - 1000COMPATIBLE WITH RFC[1000]