Difference between revisions of "Part:BBa K1638039"

 
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<partinfo>BBa_K1638039 short</partinfo>
 
<partinfo>BBa_K1638039 short</partinfo>
  
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The Hfq RNA-binding protein fused to the T25-domain of the adenylate cyclase, CyaA, from Bordetella pertussis. This construct can be used in the two-hybrid screening of protein-protein interactions between Hfq and another x protein fused to the T18 domain. If a random set of peptide aptamers is conjugated to the T18-domain, one can screen for specific peptide aptamers against Hfq.
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The Hfq RNA-binding protein fused to the T25-domain of the adenylate cyclase, CyaA, from Bordetella pertussis. This construct can be used in the two-hybrid screening of protein-protein interactions between CsrA and another x protein fused to the T18 domain. If a library of peptide aptamers is fused to the T18-domain, one can screen for specific peptide aptamers against CsrA.
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Click in on the following parts to dig deeper into the two-hybrid screening and learn how to construct your own experiment for detection of protein-protein interactions:
 +
<br>
 +
<partinfo>BBa_K1638033</partinfo>
 +
<br>
 +
<partinfo>BBa_K1638005</partinfo>
 +
 
 +
If you want to do a screening for peptide aptamers we recommend the following parts:
 +
<br>
 +
<partinfo>BBa_K1638018</partinfo>
 +
<br>
 +
<partinfo>BBa_K1638035</partinfo>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:19, 18 September 2015

Hfq fused to T25 domain of CyaA with cAMP-induced RFP reporter

The Hfq RNA-binding protein fused to the T25-domain of the adenylate cyclase, CyaA, from Bordetella pertussis. This construct can be used in the two-hybrid screening of protein-protein interactions between CsrA and another x protein fused to the T18 domain. If a library of peptide aptamers is fused to the T18-domain, one can screen for specific peptide aptamers against CsrA.

Click in on the following parts to dig deeper into the two-hybrid screening and learn how to construct your own experiment for detection of protein-protein interactions:
BBa_K1638033
BBa_K1638005

If you want to do a screening for peptide aptamers we recommend the following parts:
BBa_K1638018
BBa_K1638035


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1851
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 712
    Illegal AgeI site found at 824
  • 1000
    COMPATIBLE WITH RFC[1000]