Difference between revisions of "Part:BBa K1789022"

 
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<partinfo>BBa_K1789022 short</partinfo>
 
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This is a device which contains the sequence of the recombination of TALE1 and IAAM, the recombination of TALE2 and IAAH and scaffold 2. Through this device, we can test and verify that our scaffold can strengthen the function of IAA.
 
This is a device which contains the sequence of the recombination of TALE1 and IAAM, the recombination of TALE2 and IAAH and scaffold 2. Through this device, we can test and verify that our scaffold can strengthen the function of IAA.
  
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==Usage and Biology==
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===Usage and Biology===
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Indole-3-acetic acid (IAA), also known as auxin, is a plant hormone, which can promote growth and protect the soil from erosion. IAA can be synthesized through IAM pathway, originated from ''Pseudomonas savastanoi''. Two important enzymes are contained in this pathway, AKA IaaM and IaaH.
  
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[[File:IaaPathway.jpg]]
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In this device, the TALE1-fused IAAM and the TALE2-fused IAAH are incorporated in the E.coli due to the specific binding of TALE proteins and scaffold2, which could efficiently guide the enzymes around the DNA scaffolds, to enrich the local enzyme concentration, and promote the rate of reaction.
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==Sequence and Features==
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1789022 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1789022 SequenceAndFeatures</partinfo>
  
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==Experimental Validation==
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K1789022 parameters</partinfo>
 
<partinfo>BBa_K1789022 parameters</partinfo>
 
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Revision as of 10:04, 18 September 2015

IAA(+/+)S2

This is a device which contains the sequence of the recombination of TALE1 and IAAM, the recombination of TALE2 and IAAH and scaffold 2. Through this device, we can test and verify that our scaffold can strengthen the function of IAA.

Usage and Biology

Indole-3-acetic acid (IAA), also known as auxin, is a plant hormone, which can promote growth and protect the soil from erosion. IAA can be synthesized through IAM pathway, originated from Pseudomonas savastanoi. Two important enzymes are contained in this pathway, AKA IaaM and IaaH.

IaaPathway.jpg

In this device, the TALE1-fused IAAM and the TALE2-fused IAAH are incorporated in the E.coli due to the specific binding of TALE proteins and scaffold2, which could efficiently guide the enzymes around the DNA scaffolds, to enrich the local enzyme concentration, and promote the rate of reaction.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2724
    Illegal BamHI site found at 2311
    Illegal BamHI site found at 3669
    Illegal BamHI site found at 6072
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2431
    Illegal NgoMIV site found at 7088
    Illegal AgeI site found at 7576
    Illegal AgeI site found at 7606
    Illegal AgeI site found at 7636
    Illegal AgeI site found at 7666
    Illegal AgeI site found at 7696
    Illegal AgeI site found at 7726
    Illegal AgeI site found at 7756
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1304
    Illegal BsaI.rc site found at 5166
    Illegal BsaI.rc site found at 5370

Experimental Validation