Difference between revisions of "Part:BBa K1640022"

 
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__NOTOC__
 
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<partinfo>BBa_K1640022 short</partinfo>
 
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n/a
 
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1640022 parameters</partinfo>
 
<partinfo>BBa_K1640022 parameters</partinfo>
 
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===Overview===
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This biobrick contains a gene from the photosystem II complex of ''Chlamydomonas reinhardtii'' - psbP, with RBS included.
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This part was designed in conjunction with <html><a href="https://parts.igem.org/Part:BBa_K1640012">BBa_K1640012</a></html> and <html><a href="https://parts.igem.org/Part:BBa_K1640023">BBa_K1640023</a></html>, together they form operon 5 in our set of photosystem II operons:<br><br>
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<html><center><img src="https://static.igem.org/mediawiki/parts/thumb/a/a1/Macquarie_PS2_diagram.png/800px-Macquarie_PS2_diagram.png" width="450px" alt="PSII diagram"></center></html>
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===Biology & Literature===
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The psbP gene that comprises this part transcribes a protein that optimizes the availability of Ca2+ and Cl- cofactors in the Oxygen Evolving Complex in PSII to maintain the active Manganese cluster.
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===Protein information===
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'''psbP'''
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mass: 23.17kDa
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sequence:<br>
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MASGSDVSRRAALAGFAGAAALVSSSPANAAYGDSANVFGKVTNKSGFVPYAGDGFALLLPAKWNPSKENDF
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PGVILRYEDNFDAVNNLVVIAQDTDKKAIADFGSQDKFLESVSYLLGKQAYSGETQSEGGFAPNRVSAASLL
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DVSTTTDKKGKTYYKYELLVRSADGDEGGRHQLIGATVGSDNKLYIIKIQIGDKRWFKGAKKEAMGAFDSFT
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VV*
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===References===

Revision as of 10:02, 18 September 2015

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 537
  • 1000
    COMPATIBLE WITH RFC[1000]


Overview

This biobrick contains a gene from the photosystem II complex of Chlamydomonas reinhardtii - psbP, with RBS included.

This part was designed in conjunction with BBa_K1640012 and BBa_K1640023, together they form operon 5 in our set of photosystem II operons:

PSII diagram

Biology & Literature

The psbP gene that comprises this part transcribes a protein that optimizes the availability of Ca2+ and Cl- cofactors in the Oxygen Evolving Complex in PSII to maintain the active Manganese cluster.

Protein information

psbP

mass: 23.17kDa

sequence:
MASGSDVSRRAALAGFAGAAALVSSSPANAAYGDSANVFGKVTNKSGFVPYAGDGFALLLPAKWNPSKENDF PGVILRYEDNFDAVNNLVVIAQDTDKKAIADFGSQDKFLESVSYLLGKQAYSGETQSEGGFAPNRVSAASLL DVSTTTDKKGKTYYKYELLVRSADGDEGGRHQLIGATVGSDNKLYIIKIQIGDKRWFKGAKKEAMGAFDSFT VV*


References