Difference between revisions of "Part:BBa K1640006:Design"

 
 
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<partinfo>BBa_K1640006 short</partinfo>
 
<partinfo>BBa_K1640006 short</partinfo>
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===Design Notes===
 
===Design Notes===
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===Source===
 
  
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Genes required for photosystem II were selected using <html><a href=http://www.biocyc.org/CHLAMY/NEW-IMAGE?type=ENZYME&object=CPLX4LZ-235>biocyc entry on <i>Chlamydomonas reinhardtii</i> photosystem II complex</a>. Sequences were obtained from NCBI, targeting sequences were identified in nuclear using <a href=http://www.cbs.dtu.dk/services/ChloroP>ChloroP</a>, and removed, with an ATG added. Resulting sequences were codon optimized for e. coli expression using <a href=http://genomes.urv.es/OPTIMIZER/>Optimizer</a></html>, avoiding EcoRI, XbaI, SpeI and XbaI sites.
  
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===References===
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Ribosome binding sites were added in front of each CDS, and a promoter was added to biobricks which form the first part of their intended operon.

Latest revision as of 09:43, 18 September 2015

psbELJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 67
    Illegal AgeI site found at 173
    Illegal AgeI site found at 457
    Illegal AgeI site found at 466
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Genes required for photosystem II were selected using biocyc entry on Chlamydomonas reinhardtii photosystem II complex. Sequences were obtained from NCBI, targeting sequences were identified in nuclear using ChloroP, and removed, with an ATG added. Resulting sequences were codon optimized for e. coli expression using Optimizer, avoiding EcoRI, XbaI, SpeI and XbaI sites.

Ribosome binding sites were added in front of each CDS, and a promoter was added to biobricks which form the first part of their intended operon.