Difference between revisions of "Part:BBa K1813055"

 
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__NOTOC__
 
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<partinfo>BBa_K1813055 short</partinfo>
 
<partinfo>BBa_K1813055 short</partinfo>
  
pelB HUMCYPDB1
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Coding sequence of HUMCYPDB1 (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</a></b></html>) prefixed by the pelB localization signal.
  
 
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===Functional Parameters===
 
===Functional Parameters===
<partinfo>BBa_K1813055 parameters</partinfo>
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<partinfo>BBa_K1813046 parameters</partinfo>
 
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<h2> Background </h2>
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The pelB localization signal targets a protein it is bound to for transport to the bacterial periplasm, upon which it is cleaved off. This action can bolster the stability of some proteins, due to the relatively stronger reducing conditions prevalent in the bacterial periplasm. <br>
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This part is used in <html><b> <a href="https://parts.igem.org/Part:BBa_K1813056">BBa_K1813056</a></b></html>

Revision as of 09:32, 18 September 2015

pelB CYP2D6

Coding sequence of HUMCYPDB1 ( BBa_K1813005) prefixed by the pelB localization signal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

The pelB localization signal targets a protein it is bound to for transport to the bacterial periplasm, upon which it is cleaved off. This action can bolster the stability of some proteins, due to the relatively stronger reducing conditions prevalent in the bacterial periplasm.
This part is used in BBa_K1813056