Difference between revisions of "Part:BBa K1638013"
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<partinfo>BBa_K1638013 short</partinfo> | <partinfo>BBa_K1638013 short</partinfo> | ||
| − | This part consist of the leucine zipper region of the yeast GCN4 protein fused to the T25 domain as used in the bacterial two-hybrid system. This part allows test of functional complementation between the T18 and T25 domain. When T18-zip and T25-zip are co-transformed, the homodimerisation of the two leucine zipper will cause association of the T18 and T25 domain and catalyse formation of cAMP. By using a cAMP-induced reporter system, one can detect correct complementation. | + | This part consist of the leucine zipper region of the yeast GCN4 protein fused to the T25 domain as used in the bacterial two-hybrid system. This part allows test of functional complementation between the T18 and T25 domain. When T18-zip and T25-zip are co-transformed, the homodimerisation of the two leucine zipper will cause association of the T18 and T25 domain and catalyse formation of cAMP. By using a cAMP-induced reporter system, one can detect correct complementation [1]. |
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<partinfo>BBa_K1638013 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1638013 SequenceAndFeatures</partinfo> | ||
| + | ===References=== | ||
| + | [1]: Karimova G, Pidoux J, Ullmann A, Ladant D. A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(10):5752-6. | ||
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Revision as of 09:23, 18 September 2015
Leucine zipper fused to T25 domain of cyaA from Bordetella pertussis
This part consist of the leucine zipper region of the yeast GCN4 protein fused to the T25 domain as used in the bacterial two-hybrid system. This part allows test of functional complementation between the T18 and T25 domain. When T18-zip and T25-zip are co-transformed, the homodimerisation of the two leucine zipper will cause association of the T18 and T25 domain and catalyse formation of cAMP. By using a cAMP-induced reporter system, one can detect correct complementation [1].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 843
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1]: Karimova G, Pidoux J, Ullmann A, Ladant D. A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(10):5752-6.