Difference between revisions of "Part:BBa K1789014"
Line 19: Line 19:
  
 
Usage:
 
Usage:
−
This part is SCAF2 which is associated with a terminator. Scaf2 is used to bind TALE1 and TALE3 protein to make them fixed on the DNA. We create this part to make it more convenient every time we are required to add a scaffold.
+
This part is SCAF2 which is associated with a terminator. SCAF2 is used to bind TALE1 and TALE3 protein to make them fixed on the DNA. We create this part to make it more convenient every time we are required to add a scaffold.
  
 
Biology:
 
Biology:
 
SCAF2 is a DNA scaffold which can bind TALE protein specifically to achieve the fixing of protein or enzyme. This part is scaf2 with a terminator after it.
 
SCAF2 is a DNA scaffold which can bind TALE protein specifically to achieve the fixing of protein or enzyme. This part is scaf2 with a terminator after it.

Revision as of 09:08, 18 September 2015

SCAF2+Ter

It is the combination of scaffold system and termination.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
    Illegal AgeI site found at 180
    Illegal AgeI site found at 210
    Illegal AgeI site found at 240
    Illegal AgeI site found at 270
    Illegal AgeI site found at 300
    Illegal AgeI site found at 330
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage: This part is SCAF2 which is associated with a terminator. SCAF2 is used to bind TALE1 and TALE3 protein to make them fixed on the DNA. We create this part to make it more convenient every time we are required to add a scaffold.

Biology: SCAF2 is a DNA scaffold which can bind TALE protein specifically to achieve the fixing of protein or enzyme. This part is scaf2 with a terminator after it.