Difference between revisions of "Part:BBa K1628006"

 
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Promoter P43 is a strong promoter in ''Bacillus subtilis'' 168. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to our promoter strength assay in (showed in Figure 1), P43 is a weak promoter in ''B. amyloliquefaciens'' NK-1.
 
Promoter P43 is a strong promoter in ''Bacillus subtilis'' 168. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to our promoter strength assay in (showed in Figure 1), P43 is a weak promoter in ''B. amyloliquefaciens'' NK-1.
  
[[File:promoter3_NK.png]]
+
[[File:promoter4_NK.png]]
  
 
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Latest revision as of 09:07, 18 September 2015

P43

Promoter P43 is a strong promoter in Bacillus subtilis 168. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to our promoter strength assay in (showed in Figure 1), P43 is a weak promoter in B. amyloliquefaciens NK-1.

Promoter4 NK.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]