Difference between revisions of "Part:BBa K1628004"

 
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Promoter C2up is an artificially synthesized promoter. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to promoter strength assay in ''B. amyloliquefaciens'' NK-1 (showed in Figure 1), C2up is the strongest promoters we used in our project.
 
Promoter C2up is an artificially synthesized promoter. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to promoter strength assay in ''B. amyloliquefaciens'' NK-1 (showed in Figure 1), C2up is the strongest promoters we used in our project.
  
[[File:promoter3_NK.png]]
+
[[File:promoter4_NK.png]]
  
 
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Latest revision as of 09:04, 18 September 2015

C2up

Promoter C2up is an artificially synthesized promoter. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to promoter strength assay in B. amyloliquefaciens NK-1 (showed in Figure 1), C2up is the strongest promoters we used in our project.

Promoter4 NK.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]