Difference between revisions of "Part:BBa K1789010:Design"

 
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence.
 
  
 +
This part is designed based on the REVERSED sequence of BM2 in our project.
  
 +
We finished the construction of this part using the Golden Gate TALEN and TAL Effector Kit 2.0 by ''Addgene''. The vector was edited (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard.
 +
 +
[[File:TALE3.jpg|800px|]]
 +
 +
Fig. 1 The Sequence of TALE3 cDNA and deduced amino acids
 +
 +
 +
The protocol can be reached in our wiki's protocol page.
  
 
===Source===
 
===Source===

Revision as of 09:03, 18 September 2015


TALE3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2083
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 565
    Illegal BsaI.rc site found at 973
    Illegal BsaI.rc site found at 1279


Design Notes

This part is designed based on the REVERSED sequence of BM2 in our project.

We finished the construction of this part using the Golden Gate TALEN and TAL Effector Kit 2.0 by Addgene. The vector was edited (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard.

TALE3.jpg

Fig. 1 The Sequence of TALE3 cDNA and deduced amino acids


The protocol can be reached in our wiki's protocol page.

Source

This part is built and standardized by ourselves using GATEWAY technique.

References