Difference between revisions of "Part:BBa K1813053"

Revision as of 09:02, 18 September 2015

pelB AgCPR

Coding sequence of A. gambiae NADPH p450 reductase ( BBa_K1813010) prefixed by the pelB localization signal.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 539
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 54
Illegal AgeI site found at 337
Illegal AgeI site found at 1519
Illegal AgeI site found at 1675
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2035

Background

The pelB localization signal targets a protein it is bound to for transport to the bacterial periplasm, upon which it is cleaved off. This action can bolster the stability of some proteins, due to the relatively stronger reducing conditions prevalent in the bacterial periplasm.

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1813053 short</partinfo>
-pelB AgCPR
+Coding sequence of <i>A. gambiae</i> NADPH p450 reductase (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</a></b></html>) prefixed by the pelB localization signal.
 <!-- Add more about the biology of this part here
@@ Line 15: / Line 14: @@
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K1813053 parameters</partinfo>
+<partinfo>BBa_K1813046 parameters</partinfo>
 <!-- -->
+<h2> Background </h2>
+The pelB localization signal targets a protein it is bound to for transport to the bacterial periplasm, upon which it is cleaved off. This action can bolster the stability of some proteins, due to the relatively stronger reducing conditions prevalent in the bacterial periplasm.