Difference between revisions of "Part:BBa K1813051"

 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1813051 short</partinfo>
 
<partinfo>BBa_K1813051 short</partinfo>
  
pelB CYP6CM1
+
Coding sequence of CYP6CM1vQ (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813003">BBa_K1813003</a></b></html>) prefiexed by the pelB localization signal.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 15: Line 14:
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
<partinfo>BBa_K1813051 parameters</partinfo>
+
<partinfo>BBa_K1813046 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
<h2> Background </h2>
 +
 +
The pelB localization signal targets a protein it is bound to for transport to the bacterial periplasm, upon which it is cleaved off. This action can bolster the stability of some proteins, due to the relatively stronger reducing conditions prevalent in the bacterial periplasm.

Revision as of 08:59, 18 September 2015

CYP6G1 with pelB Expression Cassette

Coding sequence of CYP6CM1vQ ( BBa_K1813003) prefiexed by the pelB localization signal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

The pelB localization signal targets a protein it is bound to for transport to the bacterial periplasm, upon which it is cleaved off. This action can bolster the stability of some proteins, due to the relatively stronger reducing conditions prevalent in the bacterial periplasm.