Difference between revisions of "Part:BBa K1813025"
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1813024 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1813025 SequenceAndFeatures</partinfo>
  
  

Latest revision as of 08:44, 18 September 2015

CYP6G1 Expression Cassette with LacI Reversed

A construct used to achieve the bacterial expression of CYP6G1 (BBa_K1813004) under the regulative authority of the LacI repressor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2357


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP6G1 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.