Difference between revisions of "Part:BBa K1813027"
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1813024 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1813027 SequenceAndFeatures</partinfo>
  
  

Latest revision as of 08:44, 18 September 2015

CPY6CM1 Expression Cassette with LacI Reversed

A construct used to achieve the bacterial expression of CYP6CM1vQ (BBa_K1813003) under the regulative authority of the LacI repressor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP6CM1vQ without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.