Difference between revisions of "Part:BBa K1813028"
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1813024 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1813028 SequenceAndFeatures</partinfo>
  
  

Latest revision as of 08:43, 18 September 2015

LacI Reversed AgamNADPH Reductase

A construct used to achieve the bacterial expression of A. gambiae NADPH p450 reductase (BBa_K1813010) under regulative authority of the LacI repressor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BamHI site found at 1887
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1685
    Illegal AgeI site found at 2867
    Illegal AgeI site found at 3023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3383


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of A. gambiae NADPH p450 reductase without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.