Difference between revisions of "Part:BBa K1859021"
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Revision as of 08:41, 18 September 2015
Histidine tag and RFP linker [GSGSGS*2] semi-permanent generator
We designed this generator[K1859021] to express long-chain protein with keeping functionality of the protein, giving the red fluorescence.
In the study of iGEM Gifu 2014, we made long-chain protein from Circular mRNA, but it lost functionality. It is thought that folding of the protein failed for a cause．
Therefore we designed some linkers to fix the folding of the protein. Then, we combined circular parts, the 3'side of the intron [BBa_K1332005] and the 5'side of the intron [BBa_K1332003] , with those linkers and made them parts. The generator consists of Circular parts combined the linker( [BBa_K1859008] and [BBa_K1859012] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .
the generatorを導入した大腸菌は長鎖タンパク質を合成したが、そのタンパク質は赤色蛍光を呈さなかった。