Difference between revisions of "Part:BBa K1616012"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1616012 short</partinfo> | <partinfo>BBa_K1616012 short</partinfo> | ||
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ccdB comes from the ccd system in Escherichia coli F plasmid and acts as a gyrase poison. | ccdB comes from the ccd system in Escherichia coli F plasmid and acts as a gyrase poison. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This part is the reversed sequence of ccdB and can be used with a reversed promoter, reversed RBS and reversed terminator. | ||
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<partinfo>BBa_K1616012 parameters</partinfo> | <partinfo>BBa_K1616012 parameters</partinfo> | ||
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+ | ===Design Notes=== | ||
+ | Illegal sites have been checked. | ||
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+ | ===Source=== | ||
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+ | This part is the reversed sequence of the ccdB part: BBa_P1016. | ||
+ | |||
+ | ===References=== |
Latest revision as of 08:38, 18 September 2015
ccdB reversed
ccdB comes from the ccd system in Escherichia coli F plasmid and acts as a gyrase poison.
Usage and Biology
This part is the reversed sequence of ccdB and can be used with a reversed promoter, reversed RBS and reversed terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 89
Design Notes
Illegal sites have been checked.
Source
This part is the reversed sequence of the ccdB part: BBa_P1016.