Difference between revisions of "Part:BBa K1859022"
(Created page with "<html> <p> Histidine tag and RFP linker [GGSGGS*2] semi-permanent generator </p> <p> We designed this generator[K1859020] to express long-chain protein with keeping functionali...") |
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| − | Histidine tag and RFP linker [GGSGGS* | + | Histidine tag and RFP linker [GGSGGS*3] semi-permanent generator |
</p> | </p> | ||
<p> | <p> | ||
| − | We designed this generator[ | + | We designed this generator[K1859022] to express long-chain protein with keeping functionality of the protein, giving the red fluorescence. |
</p> | </p> | ||
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The generator consists of Circular parts combined the linker( | The generator consists of Circular parts combined the linker( | ||
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part: | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859009" > [BBa_K1859009] </a> |
and | and | ||
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part: | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859013" > [BBa_K1859013] </a> |
), His-RFP without stop codon | ), His-RFP without stop codon | ||
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_BBa_K1332002" >[BBa_K1332002] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_BBa_K1332002" >[BBa_K1332002] </a> | ||
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However,RFP expressed in E.coli introduced the generator is not long-chain protein. | However,RFP expressed in E.coli introduced the generator is not long-chain protein. | ||
</p> | </p> | ||
Revision as of 08:35, 18 September 2015
Histidine tag and RFP linker [GGSGGS*3] semi-permanent generator
We designed this generator[K1859022] to express long-chain protein with keeping functionality of the protein, giving the red fluorescence.
In the study of iGEM Gifu 2014, we made long-chain protein from Circular mRNA, but it lost functionality. It is thought that folding of the protein failed for a cause．
Therefore we designed some linkers to fix the folding of the protein. Then, we combined circular parts, the 3'side of the intron [BBa_K1332005] and the 5'side of the intron [BBa_K1332003] , with those linkers and made them parts. The generator consists of Circular parts combined the linker( [BBa_K1859009] and [BBa_K1859013] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .
However,RFP expressed in E.coli introduced the generator is not long-chain protein.
Linker sequence which we introduced changed structure of ribozyme related to splicing and lost their ability in function is considered as a cause.