Difference between revisions of "Part:BBa K1859020"
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Revision as of 08:30, 18 September 2015
Histidine tag and RFP linker [GGSGGS*2] semi-permanent generator
We designed this generator[K1859020] to express long-chain protein with keeping functionality of the protein, giving the red fluorescence.
In the study of iGEM Gifu 2014, we made long-chain protein from Circular mRNA, but it lost functionality. It is thought that folding of the protein failed for a cause．
Therefore we designed some linkers to fix the folding of the protein. Then, we combined circular parts, the 3'side of the intron [BBa_K1332005] and the 5'side of the intron [BBa_K1332003] , with those linkers and made them parts. The generator consists of Circular parts combined the linker( [BBa_K1859007] and [BBa_K1859011] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .
However, in RFP which Escherichia coli which I introduced this generator into developed, a long chain was not protein. However,RFP expressed in E.coli introduced the generator is not long-chain protein.
Linker sequence which we introduced changed structure of ribozyme related to splicing and lost their ability in function is considered as a cause.