Difference between revisions of "Part:BBa K1769003"
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<regulartext1>The NYMU-Taipei igem team characterize this part as an inducible promoter with GFP response as reporter. The following data was obtained by microplate reader: | <regulartext1>The NYMU-Taipei igem team characterize this part as an inducible promoter with GFP response as reporter. The following data was obtained by microplate reader: | ||
| − | <img src="https://static.igem.org/mediawiki/parts/4/4e/Promoter_test.jpg" stlye="padding-bottom:2%; padding-top:2% | + | <img src="https://static.igem.org/mediawiki/parts/4/4e/Promoter_test.jpg" stlye="padding-bottom:2%; padding-top:2%"> |
The inducible promoter was induced with different concentration(0, 1.50E-07, 6.10E-07, 2.40E-06, 9.80E-06, 3.90E-05, 1.60E-04, 6.20E-04, 1.00E-03) every 30 minutes. We started the induction when the OD600 was 0.6-0.7. The fluorescence response was measured with plate reader.<br> | The inducible promoter was induced with different concentration(0, 1.50E-07, 6.10E-07, 2.40E-06, 9.80E-06, 3.90E-05, 1.60E-04, 6.20E-04, 1.00E-03) every 30 minutes. We started the induction when the OD600 was 0.6-0.7. The fluorescence response was measured with plate reader.<br> | ||
| − | <img src="https://static.igem.org/mediawiki/parts/4/4f/Nymu-taipei-Promoter.jpg" stlye="padding-bottom:2%; padding-top:2% | + | <img src="https://static.igem.org/mediawiki/parts/4/4f/Nymu-taipei-Promoter.jpg" stlye="padding-bottom:2%; padding-top:2%"> |
The inducible promoter was induced with different concentration(0, 1.50E-07, 6.10E-07, 2.40E-06, 9.80E-06, 3.90E-05, 1.60E-04, 6.20E-04, 1.00E-03) every 30 minutes. We started the induction when the OD600 was 0.6-0.7. The fluorescence response was measured with plate reader. The red line indicate the Hill equation(P = Pmax*[SA^n]/[SA^n+Km^n] ) fit of our dose-response data with error bar showing the standard deviation and the R square value of this fit is 0.99531. "n" indicates the hill coefficient(unitless), "Pmax" indicates the maximal promoter activity, and "K" is the salicylic acid concentration while P equals 1/2*Pmax | The inducible promoter was induced with different concentration(0, 1.50E-07, 6.10E-07, 2.40E-06, 9.80E-06, 3.90E-05, 1.60E-04, 6.20E-04, 1.00E-03) every 30 minutes. We started the induction when the OD600 was 0.6-0.7. The fluorescence response was measured with plate reader. The red line indicate the Hill equation(P = Pmax*[SA^n]/[SA^n+Km^n] ) fit of our dose-response data with error bar showing the standard deviation and the R square value of this fit is 0.99531. "n" indicates the hill coefficient(unitless), "Pmax" indicates the maximal promoter activity, and "K" is the salicylic acid concentration while P equals 1/2*Pmax | ||
Revision as of 08:02, 18 September 2015
Salicylic acid sensor
This reporter is used to characterize the promoter J61051. GFP is produced when we add salicylic acid.